Occurrence of 3-amino-3,6-dideoxyhexoses in Salmonella and related bacteria.

نویسندگان

  • O Lüderitz
  • E Ruschmann
  • O Westphal
  • R Raff
  • R Wheat
چکیده

Several species of Salmonella, Citrobacter, and Arizona were examined for the presence of 3-amino sugars, which were isolated from lipopolysaccharide hydrolysates by cation exchange chromatography and identified by paper and cation exchange chromatography in several systems and by specific colorimetric procedures. 3-Amino-3,6-dideoxyglucose was identified in C. freundii 8090, C. freundii 869, S. halle, S. telaviv, S. dakar, S. wandsworth, and S. champaign; 3-amino-3,6-dideoxygalactose was found in S. tranoroa and Arizona 24. Evidence suggests that these 3-amino sugars occur in lipopolysaccharides as the N-acetyl derivatives. The composition of the lipopolysaccharides containing the 3-amino sugars was determined, and the lipopolysaccharides were compared serologically in order to correlate chemotypes with serotypes. The lipopolysaccharides containing 3-amino-3,6-dideoxyglucose reacted with serogroups 28 or 39; those containing 3-amino-3,6-dideoxygalactose were found to react with group 55 antisera. The preparation of a lipopolysaccharide from the phenol phase of the 44% aqueous phenol extraction procedure is also reported.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Characterization of 3-amino-3,6-dideoxy-D-glucose from a bacterial lipopolysaccharide.

Lipopolysaccharides were isolated from Cifrobacfer freundii ATCC 8090 by 44% aqueous-phenol fractionation. The lipopolysaccharides were isolated from the phenol phase and phenol-water interphase fractions. Acid hydrolysis yielded glucosamine and a 3-amino sugar identified as 3amino-3,6-dideoxyglucose by comparison with authentic 3-amino-3,6-dideoxyhexoses by ion exchange chromatography. Compari...

متن کامل

CDP-6-deoxy-delta 3,4-glucoseen reductase from Yersinia pseudotuberculosis: enzyme purification and characterization of the cloned gene.

The 3,6-dideoxyhexoses, usually confined to the cell wall lipopolysaccharide of gram-negative bacteria, are essential to serological specificity and are formed via a complex biosynthetic pathway beginning with CDP-D-hexoses. In particular, the biosynthesis of CDP-ascarylose, one of the naturally occurring 3,6-dideoxyhexoses, consists of five enzymatic steps, with CDP-6-deoxy-delta 3,4-glucoseen...

متن کامل

Genetic and structural relationships of Salmonella O55 and Escherichia coli O103 O-antigens and identification of a 3-hydroxybutanoyltransferase gene involved in the synthesis of a Fuc3N derivative.

O-antigen (O-polysaccharide), a part of the outer membrane of Gram-negative bacteria, is one of the most variable cell constituents and is related to bacterial virulence. O-antigen diversity is almost entirely due to genetic variations in O-antigen gene clusters. In this study, the O-polysaccharide structures of Salmonella O55 and Escherichia coli O103 were elucidated by chemical analysis and n...

متن کامل

Enzymatic Synthesis of Cytidine Diphosphate 3,6=Dideoxyhexoses IV. PARTIAL RESOLUTION OF THE ENZYMES REQUIRED FOR REDUCTION OF CYTIDINE DIPHOS- PHATE 4-KETO-6-DEOXY-n-GLUCOSE AND ISOLATION OF A NEW INTERMEDIATE*

The enzymatic system for the formation of @dine diphosphate 3,6dideoxyhexose from CDP-4-keto-6-deoxy-nglucose has been separated into three protein fractions. Two of these fractions, El and E3, participate in a TPNHrequiring reaction in which a new cytidine nucleotide intermediate is formed. Since this intermediate can be reduced chemically to yield 3,6-dideoxy-n-galactose (abequose) and 3,6-di...

متن کامل

Preliminary Report of NAD+-Dependent Amino Acid Dehydrogenase Producing Bacteria Isolated from Soil

Amino acid dehydrogenases (L-amino acid: oxidoreductase deaminating EC 1.4.1.X) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either NAD+, NADP+ or FAD. These enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kit...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of bacteriology

دوره 93 5  شماره 

صفحات  -

تاریخ انتشار 1967